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KMID : 0624620160490050297
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BMB Reports
2016 Volume.49 No. 5 p.297 ~ p.302
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Transduced Tat-DJ-1 protein inhibits cytokines-induced pancreatic RINm5F cell death
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Jo Hyo-Sang
Yeo Hyeon-Ji
Cha Hyun-Ju
Kim Sang-Jin
Cho Su-Bin
Park Jung-Hwan
Lee Chi-Hern
Yeo Eun-Ji
Choi Yeon-Joo
Eum Won-Sik
Choi Soo-Young
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Abstract
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Loss of pancreatic β-cells by oxidative stress or cytokines is associated with diabetes mellitus (DM). DJ-1 is known to as a multifunctional protein, which plays an important role in cell survival. We prepared cell permeable wild type (WT) and mutant type (M26I) Tat-DJ-1 proteins to investigate the effects of DJ-1 against combined cytokines (IL-1β, IFN-γ and TNF-α)-induced RINm5F cell death. Both Tat-DJ-1 proteins were transduced into RINm5F cells. WT Tat-DJ-1 proteins significantly protected against cell death from cytokines by reducing intracellular toxicities. Also, WT Tat-DJ-1 proteins markedly regulated cytokines-induced pro- and anti-apoptosis proteins. However, M26I Tat-DJ-1 protein showed relatively low protective effects, as compared to WT Tat-DJ-1 protein. Our experiments demonstrated that WT Tat-DJ-1 protein protects against cytokine-induced RINm5F cell death by suppressing intracellular toxicities and regulating apoptosisrelated protein expression. Thus, WT Tat-DJ-1 protein could potentially serve as a therapeutic agent for DM and cytokine related diseases.
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KEYWORD
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Cytokines, Protein therapy, Tat-DJ-1, Toxicity, Wild type and mutants
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